anti 11β hsd1  (Proteintech)

Journal: Frontiers in Endocrinology

Article Title: Gut microbiota is involved in the exacerbation of adrenal glucocorticoid steroidogenesis in diabetic animals by activation of the TLR4 pathway

doi: 10.3389/fendo.2025.1555203

Figure Lengend Snippet: Suppression of TLR4 signaling by TAK-242 represses expression of the steroidogenic machinery, TLR4, and TRIF in the adrenal glands of diabetic mice. Seven days after diabetes induction, animals were treated with the TLR4 antagonist TAK-242 (3 mg/kg, i.p.) once a day for 14 consecutive days. Control animals received an equal amount of vehicle (0.1% DMSO). Analyses were performed 21 days after diabetes induction. (A) Expression of StAR, 11βHSD1, and MC2R in the adrenal glands. Expression of TLR4 (B) and TRIF (C) in the adrenal glands. Protein expression was determined by western blot. Data were normalized to β-actin and represented as ratios between target protein levels relative to controls. Each bar represents the mean ± standard error of the mean. Pink dots represent the number of animals analyzed. Statistical analyses were performed by one-way ANOVA followed by Newman–Keuls test. * p<0.05. ** p<0.005. ns, non-significant; 11βHSD1, 11β-Hydroxysteroid dehydrogenase type 1; MC2R, melanocortin receptor 2; StAR, steroidogenic acute regulatory protein; TAK, TAK-242; TLR4, toll-like receptor 4; TRIF, TIR-domain-containing adapter-inducing interferon-β.

Article Snippet: Primary antibodies against the following proteins were used: anti-11β-HSD1 (1:200; Santa Cruz Biotechnology), anti-MC2R (1:200; Santa Cruz Biotechnology), anti-StAR (1:250; Santa Cruz Biotechnology), anti-TLR4 (1:250; Santa Cruz Biotechnology), and anti-TRIF (1:200; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Expressing, Control, Western Blot